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2.5× stop buffer  (Promega)

 
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    Promega 2.5× stop buffer
    2.5× Stop Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2%2E5%C3%97+stop+buffer/2+5%C3%97+stop+buffer/pmc10674617-278-18-38
    Average 90 stars, based on 1 article reviews
    2.5× stop buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Design, Synthesis, Evaluation and Molecular Dynamics Simulation of Dengue Virus NS5-RdRp Inhibitors
    Article Snippet: After a reaction time of 120 min, the process was terminated by adding 10 μL of a 2.5× STOP buffer, comprising 200 mM NaCl, 25 mM MgCl 2 , and 1.5 M DEA adjusted to pH 10 using Promega.

    Article Title: NS5 from Dengue Virus Serotype 2 Can Adopt a Conformation Analogous to That of Its Zika Virus and Japanese Encephalitis Virus Homologues
    Article Snippet: At the time points indicated above, 10 μl of 2.5× stop buffer (200 mM NaCl, 25 mM MgCl 2 , 1.5 M diethanolamine, pH 10; Promega) with 25 nM calf intestinal alkaline phosphatase (CIP; New England Biolabs) was added to the wells to terminate the reactions.

    Article Title: A Crystal Structure of the Dengue Virus Non-structural Protein 5 (NS5) Polymerase Delineates Interdomain Amino Acid Residues That Enhance Its Thermostability and de Novo Initiation Activities
    Article Snippet: At the indicated time points, 30 μl of 2.5× STOP buffer (200 m m NaCl, 25 m m MgCl 2 , 1.5 m diethanolamine, pH 10; Promega) with 25 n m calf intestinal alkaline phosphatase (New England Biolabs) was added to the wells to terminate the reactions.

    Article Title: A Crystal Structure of the Dengue Virus NS5 Protein Reveals a Novel Inter-domain Interface Essential for Protein Flexibility and Virus Replication
    Article Snippet: At the indicated time-points, 10 μl of 2.5× STOP buffer (200 mM NaCl, 25 mM MgCl 2 , 1.5 M DEA, pH 10; Promega) with 25 nM calf intestinal alkaline phosphatase (CIP; New England Biolabs) was added to the wells to terminate the reactions.

    Article Title: Design, Synthesis, Evaluation and Molecular Dynamics Simulation of Dengue Virus NS5-RdRp Inhibitors.
    Article Snippet: After a reaction time of 120 min, the process was terminated by adding 10 μL of a 2.5× STOP buffer, comprising 200 mM NaCl, 25 mM MgCl2, and 1.5 M DEA adjusted to pH 10 using Promega.

    Article Title: A crystal structure of the Dengue virus NS5 protein reveals a novel inter-domain interface essential for protein flexibility and virus replication.
    Article Snippet: At the indicated time-points, 10 μl of 2.5× STOP buffer (200 mMNaCl, 25 mMMgCl2, 1.5 M DEA, pH 10; Promega) with 25 nM calf intestinal alkaline phosphatase (CIP; New England Biolabs) was added to the wells to terminate the reactions.

    Incubation:

    Article Title: Activation of Peripheral Blood Mononuclear Cells by Dengue Virus Infection Depotentiates Balapiravir
    Article Snippet: .. The plate was sealed, shaken, centrifuged, and incubated at room temperature for 90 min. For terminating the reaction, 20 μl of 2.5× Stop buffer (200 mM NaCl, 25 mM MgCl 2 , 1.5 M deoxyethanolamine (DEA), pH 10; Promega) containing 25 nM calf intestinal alkaline phosphatase (CIP; NEB) was added to the reaction mixtures. ..



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